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JetPRIME™ DNA & siRNA Transfection Reagent
JetPRIME™ DNA & siRNA Transfection Reagent
JetPrime transfection reagent can now be used for DNA and siRNA co-transfection.jetPRIME™ is a new versatile and powerful DNA and siRNA transfection reagent for day-to-day experiments.
jetPRIME™ ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. Hence, jetPRIME™ is ideal for DNA/siRNA co-transfection. jetPRIME™ is very gentle to cells since it requires low amounts of nucleic acid and reagent during transfection.
1.5 ml is sufficient to perform ca. 375 transfections in 6-well plates.
Outstanding DNA transfection efficiency Using jetPRIME™ reagent, DNA transfection efficiencies range between 70 and 90% on adherent cell lines, using only 2 µg of DNA and 4 µl of reagent per well in 6-well plates (Fig. 1-2).
Fig. 1. Transfection efficiency assessed by FACS analysis in various cell lines 24h following transfection of 2 µg pCMV-GFP plasmid and 4 µl jetPRIME™ per well in 6-well plates.
Fig. 2. GFP expression in HEK-293 and SK-OV-3 cells 24 h after transfection using 2 µg pCMV-GFP plasmid and 4 µl of jetPRIME™ in
6-well plates.
Using jetPRIME™ reagent and DNA, transfection efficiencies range between 70 and 90 % on standard cell lines.
Economical: less DNA and less reagent needed jetPRIME™ is such a powerful in vitro transfection reagent that it only requires a small amount of plasmid DNA and reagent (Fig. 3). As a result, it is a very economical reagent.
Fig. 3. Amounts of DNA and reagent (jetPRIME™ and competitor) added per well for transfection according to manufacturers’ recommendations.
In addition to reducing costs, using less DNA also minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME™ is the reagent of choice for high transfection efficiency with excellent cell viability.
Better cell viability jetPRIME™ is extremely gentle to cells during transfection leading to increased cell viability (Fig. 4) and improved transfection results. Cells transfected with jetPRIME™ are healthy, while major cytotoxicity is observed with competitor.
Fig. 4. Phase contrast microscopy of HeLa cells 24 h after transfections performed according to the manufacturer’s recommendations for each reagent.
Excellent gene silencing jetPRIME™ leads to over 90% knockdown of endogenous gene expression in a variety of cell lines. For example, jetPRIME™-mediated transfection of HeLa cells with10 nM siRNA duplexes targeting endogenous lamin A/C in HeLa cells drastically reduces lamin A/C gene expression to barely detectable level (Fig. 5).
Fig. 5. Endogenous lamin A/C silencing using jetPRIME™. HeLa cells were transfected with 10 nM of 21-mer lamin A/C siRNA. After 48 h, lamin A/C silencing was assessed by immunofluorescence microscopy using an antibody against lamin A/C.
Co-transfection of DNA & siRNA jetPRIME™ is well suited for DNA and siRNA co-transfection experiments. It shows highly efficient gene silencing in a variety of cell lines with very low toxicity. Over 90% silencing is achieved in adherent cells, using 10 nM siRNA (Fig. 6).
Fig. 6. Exogenous luciferase gene silencing in several cell lines after DNA & siRNA co-transfection using jetPRIME™ performed with 400 ng pCMV-Luc and 10 nM of luciferase siRNA per well in 6-well plates.
Convenient protocol jetPRIME™ is an easy-to-use transfection reagent
(Fig. 7):
• Fast and easy to scale up and down
• Compatible with serum and antibiotics
Fig. 7. jetPRIME™ convenient protocol for DNA, siRNA and co-transfection of DNA and siRNA.
