C-chip - cell counting with new disposable counting chambers
High-precision Neubauer counting chamber “improved”, suitable for any cell type, minimalises infection risk.
High-precision Neubauer counting chamber “improved”, suitable for any cell type.
The counting chamber looks like the familiar Neubauer "improved" hemocytometer: The cells are distributed over 3 x 3 large squares, each with 1 mm edge length and with a surface area of 1 mm². Count your cells as usual – whether stained or unstained.
With the C-Chip, you inject the sample, stained or unstained, into the desired chamber. Two separate counting chambers facilitate two counts per C-Chip.
Quick, easy and safe:
- Minimal manufacturing tolerances
- High precision
- Small risk of infection
Easy to use:
1. Mix the samples well
2. Load 10 μl of sample into the sample injection area so that it fills the chamber by capillary action
3. Count the cells under microscope
Account of the cells:
Cells per ml = Average count per square x dilution factor x volume factor
Erythrocyte counting (1:200 dilution)
1. Dilute blood using accepted laboratory methods
2. Load 10 μl of diluted sample into the sample injection area
3. Count the erythrocytes in the 5 small squares (four small corner squares and one small middle square) of the large center square
Erythrocytes per ml = cells in 5 small squares x 5 x 200 (dilution factor) x 10000 (volume factor)
Mammalian cell counting
1. Treat the cell samples with Trypsin-EDTA.
2. Carefully remove the supernatant with a pipette tip without disturbing the pellet
3. Add an appropriate volume of growth media or PBS to dilute to a final concentration of 5x103 cells/ml to 5x106 cells per ml
4. Thoroughly resuspend the cell pellet with a pipette
5. Check visually if there are any cell clumps or agglomerates
6. Load 10 μl of sample into the sample injection area
7. Count the cells in 5 large squares
Cells per ml = (cells in 5 large squares/5) x dilution factor x 10000 (volume factor)
Leukocyte counting (1:20 dilution)
1. Dilute blood using accepted laboratory methods
2. Load 10 μl of diluted sample into the sample injection area
3. Count the leukocytes in the 4 large corner squares
Leukocytes per ml = (cells in 4 corner squares/ 4) x 20 (dilution factor) x 10000 (volume factor)
DHC-N01, 50pcs Volejte Kč
DHC-N01, 10x50pcs Volejte Kč
DHC-N01, 30x50pcs Volejte Kč
